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3 publications in Science

BLUEPRINT Sept 2014 kl
In the September issue of Science, 3 papers from the BLUEPRINT consortium are published. BLUEPRINT is a large-scale research project receiving close to 30 million euro funding from the EU. Henk Stunnenberg is coordinating this consortium consisting of 39 leading European universities, research institutes and industry entrepreneurs. All 3 papers describe research which had an important input from the department of Molecular Biology.
1. mTOR- and HIF-1α–mediated aerobic glycolysis as metabolic basis for trained immunity
2. Transcriptional diversity during lineage commitment of human blood progenitors
3. Epigenetic programming of monocyte-to-macrophage differentiation and trained innate immunity

RIMLS Lecture Series: Dirk Schübeler – December 18 (16.00, 8th floor RIMLS)

On December 18th, 2014, Dirk Schubeler will give a presentation entitled “The genetics of epigenetics” at 16.00 in the Figdor Lecture Theatre on the 8th floor of the RIMLS building. Following the lecture, drinks and snacks will be available to socialise with the lecturer and collegues.

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Dirk Schübeler is a Senior Group Leader at the Friedrich Miescher Institute for Biomedical Research in Basel. His group studies the role of epigenetic modifications of DNA and nucleosomes in transcriptional regulation and genome maintenance. A particular interest is the role of epigenetic regulation in stabilizing transcriptional programs in defined cellular states and how it relates to the developmental potential of a given cell. The regulation of pluripotency of stem cells and their epigenetic reprogramming during differentiation provide important models to address these questions. To understand how stem cell maintenance and loss of pluripotency are regulated via chromatin and DNA methylation the group develops and applies functional genomics approaches. With these quantitative measures of the epigenome are performed to identify regulatory patterns and their function in cell fate decisions.

Dirk Schübeler is an elected member of the European Network of Excellence “The epigenome”, an EMBO Young Investigator and member of the Swiss Systems Biology initiative on Cell Plasticity.

Seminar Pim Huis in ‘t Veld – December 16, 2014 (13.00, 8th floor RIMLS)

On December 16th, 2014 12:00 hrs. – 13:00 dr. Pim Huis in ‘t Veld, IMP, Research Institute of Molecular Pathology, Vienna, Austria will give a seminar on “Characterization of a DNA exit gate in the human cohesion ring” in the Figdor Lecture Theatre, 8th floor RIMLS Building.

Chromosome segregation depends on sister chromatid cohesion mediated by cohesin. Cohesin’s Smc1, Smc3 and Scc1 subunits form tripartite rings that are thought to open at distinct sites to allow entry and exit of DNA. However, direct evidence for the existence of open forms of cohesin is lacking. Here we show that cohesin’s proposed DNA exit gate is formed by interactions between Scc1 and the coiled-coil region of Smc3. Mutation of this interface abolished cohesin’s ability to stably associate with chromatin and to mediate cohesion. Electron microscopy revealed that weakening of the Smc3-Scc1 interface, as well as proteolytic cleavage of Scc1, resulted in opening of cohesin rings. These open forms may resemble intermediate states of cohesin normally generated by the release factor Wapl and the protease separase, respectively.

Seminar Duncan Odom – December 9, 2014 (14.00, 8th floor RIMLS)

As part of the ‘Molecular Principles of Development and Disease’ seminar series, Dr. Duncan Odom is visiting our departments.
He will meet with the PhD students over lunch and will present a seminar on ‘Enhancer evolution in twenty mammals’ at 14:00 on the 8th floor lecture theatre of the RIMLS building.

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Duncan Odom, group leader, Cambridge Research Institute, Cambridge, UK, compares how transcription and transcriptional regulation vary during evolution, and the implications this regulatory plasticity has for diseases such as cancer. His team uses numerous new high-throughput methods combined with analysis of multiple mammals and vertebrates to reveal fundamental biological insights into tissue-specification and genome evolution.

Postdoctoral Position in Molecular Carcinogenesis (1,0 fte)

This position has been filled

Faculty of Science
Maximum salary: € 3,831 gross/month
Vacancy number: 62.39.14
Application deadline: 29 June 2014

Responsibilities
Recurring chromosomal abnormalities have been identified in a variety of cancers, but are most frequently associated with haematological malignancies such as acute myeloid leukaemia (AML). These cytogenetic changes often cause gene fusions leading to expression of chimeric oncofusion proteins.
As a postdoctoral researcher in Molecular Carcinogenesis you will focus on unravelling the role of these oncofusion proteins in carcinogenesis. Your aim will be to identify the binding regions and transcriptional consequences associated with oncofusion protein expression as well as identifying the alterations within the associated protein complex during leukaemogenesis. You will use next-generation sequencing as well as mass spectrometry to identify oncofusion protein target sites and protein complex composition, which then will be further characterized structurally as well as functionally in relation to the aetiology of acute myeloid leukaemia.

Work environment
Research at the department of Molecular Biology aims at unravelling the molecular basis of development and differentiation emanating from the genome and epigenome in the context of health and disease with an emphasis on cancer. State-of-the-art technologies are applied ranging from single molecule studies to genome-wide elucidation of genetic and epigenetic pathways and mechanisms. A Next Generation Sequencing platform is operational in the department to analyse transcription factor binding sites, epigenetic marks, DNA-methylation and RNA-seq at a comprehensive genome-wide scale. High-accuracy mass spectrometric approaches are used to identify and to characterize protein complexes. In-depth analysis of the data is performed and supported by our own bioinformatic team as well as with the help of outside collaborators. Moreover, the group participates in a number of international consortia tackling pathways and molecular mechanisms in lineage commitment of (embryonic) stem cells and pluripotency, nuclear receptor action and cancer.

What we expect from you
– PhD degree in Biomedical Sciences/Biology/Molecular Life Sciences/Bioinformatics;
– experience in molecular biology, cell biology and mass spectrometry;
– experience in iPS cell culture;
– strong affinity with fundamental research related to human disease;
– motivated to perform cutting-edge research;
– well-developed social and communication skills;
– accurate and well-structured working style.

What we have to offer
We offer you:
– employment: 1,0 fte;
– a maximum gross monthly salary of € 3,831 based on a 38-hour working week (salary scale 10);
– in addition to the salary: an 8% holiday allowance and an 8.3% end-of-year bonus;
– duration of the contract: temporary (2,5 years);
– you will be classified as a Postdoctoral Researcher 4 in the Dutch university job-ranking system (UFO).

Are you interested in our excellent employment conditions?

Would you like to know more?
Further information on: The Radboud Institute for Molecular Life Sciences (RIMLS)

Dr. Ir. Joost Martens, assistant professor
E-mail: j.martens@ncmls.ru.nl

Applications
Are you interested?
Please include with your application a motivation letter (attn. of ms. W. van der Pluijm), CV and any required attachments. You should upload these documents using ‘Apply directly‘.
Interested candidates should send a letter of motivation with full curriculum vitae and contact information of at least two references.
For more information on the application procedure: +31 (0)24 3652131

Please note: We will only consider applications submitted via our application form (which is accessed by clicking the ‘Apply directly‘ link).

No commercial propositions please.